Fast Analysis of Biological Compounds by Gradient Liquid Chromatography Using Pillar Array Column

Here we describe fast analysis of biomolecules by newly developed pressure-driven liquid chromatography (LC) on a chip with integration of a gradient elution system. We have reported the fabrication of a gradient elution system using a cross-Tesla mixer on a chip with pillar array columns [1]. In this study, we applied the chip to the analysis of biological compounds, and showed that 10 times faster separation was achieved under gradient elution than under isocratic elution. The retention times under gradient elution conditions were corresponded to the values estimated by semi-empirical equation, which showed that the gradient elution system worked validly.